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Equal amounts of protein (30 g per well) were separated in Mini-PROTEAN TGX Stain Free precast gels (Bio-Rad, 4868096), and transferred to nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad)
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They were maintained in Allentown XJ cages (34 per cage) with Andersons Enrich-o-cob bedding (Maumee, OH) under controlled temperature (21 C) and lighting conditions (14-h light/10-h dark cycle for breeding
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